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Proceedings Paper

AFM analysis of the formation of DNA aggregates on polymeric biochips
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Paper Abstract

The demand for polymer-based DNA microarrays will increase because of their cost-effectiveness, biocompatibility and easy processing. However not all polymers are ideal substrates because of different chemical interactions of polymeric substrates with the DNA molecules. Results from AFM analysis of DNA immobilised on polymeric surfaces are evaluated using fractality, Gaussian distribution and lateral force imaging. It has been found that the nanosize defects in the substrate, such as poly-l-lysine, plays an important role in the growth of DNA aggregates in a vertical direction, whereas the covalent binding of DNA molecules on NHS-functionalised cyclo-olefin copolymer leads to the lateral growth of DNA aggregates.

Paper Details

Date Published: 16 February 2005
PDF: 7 pages
Proc. SPIE 5651, Biomedical Applications of Micro- and Nanoengineering II, (16 February 2005); doi: 10.1117/12.584142
Show Author Affiliations
Prashant D. Sawant, Swinburne Univ. of Technology (Australia)
Gregory Watson, Griffith Univ. (Australia)
Sverre Myhra, Griffith Univ. (Australia)
Dan V. Nicolau, Swinburne Univ. of Technology (Australia)


Published in SPIE Proceedings Vol. 5651:
Biomedical Applications of Micro- and Nanoengineering II
Dan V. Nicolau, Editor(s)

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