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Proceedings Paper

Miniature fluorescence detection system for protein chips
Author(s): Hoseong Kim; Jaeho Choi; Kook-Nyung Lee; Yongkwon Kim
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Paper Abstract

We report the development of miniature fluorescence detection systems that employ miniature prism, mirrors and low cost CCD camera to detect the fluorescence emitted from 40 fluorescently-labeled protein patterns without scanner. This kind of miniature fluorescence detection systems can be used in point of care. We introduce two systems, one uses prism + mirror block and the other uses prism and two mirrors. A large NA microscope eyepiece and low cost CCD camera are used. We fabricated protein chip containing multi-pattern BSA labeled with Cy5, using MEMS technology and modified the surface chemically to clean and to immobilize proteins. The measurements show that the combination of prism and mirrors can homogenize elliptical excitation light over the sample with higher optical efficiency, and increase the separation between excitation and fluorescence light at the CCD to give higher signal intensity and higher signal to noise ratio. The measurements also show that protein concentrations ranging from 10 ng/ml to 1000 ng/ml can be assayed with very small error. We believe that the proposed fluorescence detection system can be refined to build a commercially valuable hand-held or miniature detection device.

Paper Details

Date Published: 22 January 2005
PDF: 11 pages
Proc. SPIE 5718, Microfluidics, BioMEMS, and Medical Microsystems III, (22 January 2005); doi: 10.1117/12.583281
Show Author Affiliations
Hoseong Kim, Chung Ang Univ. (South Korea)
Jaeho Choi, Chung Ang Univ. (South Korea)
Kook-Nyung Lee, Seoul National Univ. (South Korea)
Yongkwon Kim, Seoul National Univ. (South Korea)


Published in SPIE Proceedings Vol. 5718:
Microfluidics, BioMEMS, and Medical Microsystems III
Ian Papautsky; Isabelle Chartier, Editor(s)

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