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Proceedings Paper

Development of a new optical fiber epifluorescence biosensor system
Author(s): Lianwei Jiang; Argyrios Margaritis; Nils O. Petersen
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Paper Abstract

This paper describes the development of a novel biosensor system for competitive binding immunofluorescence assay. The system is based on a specially designed fluorescence microscope combined with optical fibers. Protein A is covalently immobilized on the activated sensing tip surface of a quartz fiber or on the activated surface of glass cover slips. The surface is then incubated with rabbit IgG to reach a reasonable binding density and homogeneous distribution for adequate detectability. The sensor is exposed to FITC labeled and unlabeled goat anti-rabbit IgG. Fluorescence of sensor bound analyte, excited by an Argon ion laser 476 nm line and emitted from the sensing tip or sensing spot on the sample slips, is coupled back into the fiber and detected by the highly sensitive sensing system. The fluorescence signal is inversely proportional to the concentration of unlabeled anti-rabbit IgG in the sample. Sensor's limitation of detection has been lowered to a concentration level of 10-10 M and 10 - 20 (mu) l sample volume. Typically, 6 f mol of unlabeled antibody in a 15 (mu) l sample volume and with a 10 - 20 min incubation period, could be measured.

Paper Details

Date Published: 16 April 1992
PDF: 11 pages
Proc. SPIE 1648, Fiber Optic Medical and Fluorescent Sensors and Applications, (16 April 1992); doi: 10.1117/12.58301
Show Author Affiliations
Lianwei Jiang, Univ. of Western Ontario (Canada)
Argyrios Margaritis, Univ. of Western Ontario (Canada)
Nils O. Petersen, Univ. of Western Ontario (Canada)


Published in SPIE Proceedings Vol. 1648:
Fiber Optic Medical and Fluorescent Sensors and Applications
Douglas R. Hansmann; Fred P. Milanovich; Gerald G. Vurek; David R. Walt, Editor(s)

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