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Proceedings Paper

Chelation-enhanced fluorescence detection of metal and nonmetal ions in aqueous solution
Author(s): Anthony W. Czarnik
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Paper Abstract

Our group has previously described large chelation-enhanced fluorescence (CHEF) effects upon the binding of metal ions, phosphates, and carboxylates to conjugate probes, providing large, readily measurable signals to these molecular recognition events. In understanding the structural requirements for CHEF, it is now possible to use the vast body of information on selective binding by azacrowns and cryptands in the synthesis of selective fluorescence probes. For example, a conjugate probe that allows for the selective, simultaneous assay of Zn(II) and Cd(II) ions has been synthesized and is described. In the homologous series of anthrylazamacrocycles that demonstrate chelation-enhanced fluorescence (CHEF) upon Zn(II) or Cd(II) binding in water, the pentacyclen derivative uniquely complexes Cd(II) with perturbation of the emission spectrum. The binding of anions such as phosphate and citrate give rise to fluorescence enhancements as large as six-fold; an observed pH dependence on the magnitude of fluorescence enhancements upon phosphate binding points to intracomplex protonation of the benzylic nitrogen by the HPO42- ion as the origin of this CHEF effect. Anthrylpolyamine conjugate probes yield large (up to 80-fold) changes in fluorescence upon binding to biological polyanions (e.g., DNA, heparin, and polyglutamate) at 1 M concentrations. These fluorescence changes have been used as the basis for a fluorometric assay of heparinase activity; the enzymatic hydrolysis of ATP can also be monitored conveniently using anthrylpolyamine fluoroionophores.

Paper Details

Date Published: 16 April 1992
PDF: 17 pages
Proc. SPIE 1648, Fiber Optic Medical and Fluorescent Sensors and Applications, (16 April 1992); doi: 10.1117/12.58297
Show Author Affiliations
Anthony W. Czarnik, Ohio State Univ. (United States)

Published in SPIE Proceedings Vol. 1648:
Fiber Optic Medical and Fluorescent Sensors and Applications
Douglas R. Hansmann; Fred P. Milanovich; Gerald G. Vurek; David R. Walt, Editor(s)

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