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Proceedings Paper

Fluorescence-lifetime-based sensing of blood gases and cations
Author(s): Joseph R. Lakowicz; Henryk Szmacinski; Klaus W. Berndt
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Paper Abstract

Chemical sensing with fluorescent probes is usually accomplished by measuring the fluorescence intensity or using wavelength-ratiometric probes. Measurement of fluorescence decay times, rather than intensities, is advantageous because the decay times are largely independent of intensity changes due to light losses, photobleaching, or probe washout. Present technology allows measurement of nanosecond decay times by the phase-modulation method with inexpensive and robust instrumentation. This report describes the use of phase- modulation fluorometry for lifetime-based sensing of O2, pH, Ca2+ and K+.

Paper Details

Date Published: 16 April 1992
PDF: 14 pages
Proc. SPIE 1648, Fiber Optic Medical and Fluorescent Sensors and Applications, (16 April 1992); doi: 10.1117/12.58296
Show Author Affiliations
Joseph R. Lakowicz, Univ. of Maryland School of Medicine (United States)
Henryk Szmacinski, Univ. of Maryland School of Medicine (United States)
Klaus W. Berndt, Univ. of Maryland School of Medicine (United States)


Published in SPIE Proceedings Vol. 1648:
Fiber Optic Medical and Fluorescent Sensors and Applications
Douglas R. Hansmann; Fred P. Milanovich; Gerald G. Vurek; David R. Walt, Editor(s)

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