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Proceedings Paper

Detection of volatile and soluble general anesthetics using a fluorescence-based fiber optic sensor: recent progress in chemical sensitivity and noise sources
Author(s): Paul Yager; Susan B. Abrams
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Paper Abstract

A fiber optic sensor for general anesthetics based on the phase transition of immobilized phospholipid vesicles is under development. Current work centers on evaluating the sensor response to different anesthetics and instrumentation design. The fluorescence of laurdan- doped liposomes is found to respond linearly to the infusible anesthetics thiopental sodium and Propofol. Preliminary experiments have been performed to determine sources of noise in the optical and electronic components of the sensor as it is now configured. One potential noise source is the liposome sample at the fiber tip; photobleaching and thermal fluctuations due to heating by the illuminating 360 nm radiation can affect measurement of the anesthetic level. Heating of the sample is a factor at high illumination levels, but photobleaching, which reduces the signal intensity, does not alter the intensity ratio upon which the anesthetic concentration measurement is based. Optical microscopy of fiber tips embedded in liposomes allows direct observation of the light intensity near the tip of the fiber despite the extreme turbidity of the suspension. Light intensity drops to less than 10% of its maximum intensity at the fiber tip within 300 micrometers . Further use of this technique should allow monitoring the effects of photobleaching on the spatial distribution of the liposomes responsible for the measured optical signal.

Paper Details

Date Published: 16 April 1992
PDF: 12 pages
Proc. SPIE 1648, Fiber Optic Medical and Fluorescent Sensors and Applications, (16 April 1992); doi: 10.1117/12.58287
Show Author Affiliations
Paul Yager, Univ. of Washington (United States)
Susan B. Abrams, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 1648:
Fiber Optic Medical and Fluorescent Sensors and Applications
Douglas R. Hansmann; Fred P. Milanovich; Gerald G. Vurek; David R. Walt, Editor(s)

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