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Proceedings Paper

Automated immunofluorescence measurement system using a bleaching method
Author(s): Yanmin Tao; G. Boeck; Norbert H. Nessler; Erwin Hochmair
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Paper Abstract

The bleaching process, which is the fading of the fluorescence of dye molecules under continuous excitation, can be used to analyze the specificity of the antibody-antigen reaction in immunological tests. The automated immunofluorescence measurement system based on the analysis of bleaching characteristics, which can be described by the formfactor beside the maximum fluorescence intensity, has been developed. These two parameters can be used in fluorescence imaging to detect rare events among nonspecific staining.

Paper Details

Date Published: 1 April 1992
PDF: 6 pages
Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); doi: 10.1117/12.58280
Show Author Affiliations
Yanmin Tao, Univ. Innsbruck (Austria)
G. Boeck, Univ. Innsbruck (Austria)
Norbert H. Nessler, Univ. Innsbruck (Austria)
Erwin Hochmair, Univ. Innsbruck (Austria)


Published in SPIE Proceedings Vol. 1640:
Time-Resolved Laser Spectroscopy in Biochemistry III
Joseph R. Lakowicz, Editor(s)

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