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Proceedings Paper

Models for fluorescence energy transfer between moving donors and acceptors
Author(s): B. Wieb Van der Meer; Matthew A. Raymer; Shawn L. Wagoner; Richard L. Hackney; Joseph M. Beechem; Enrico Gratton
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Paper Abstract

A number of theoretical models are introduced in which donors and acceptors perform lateral or rotational motion during the time in which fluorescence energy transfer takes place. Each case is reduced to an eigenvalue problem, and for each model calculations are made of observables, such as fluorescence intensities and anisotropies, by employing matrix methods. It is shown that the observables depend on the size of the motional step only if fluorescence energy transfer occurs. This finding indicates that fluorescence energy transfer studies may reveal whether the dynamics of a system (e.g., a protein) is better described in terms of transitions between a number of discrete states or in terms of diffusion equations. Some theoretical tools are offered for analyzing fluorescence energy transfer data without restrictive assumptions for motional averaging regimes or the orientation factor.

Paper Details

Date Published: 1 April 1992
PDF: 10 pages
Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); doi: 10.1117/12.58266
Show Author Affiliations
B. Wieb Van der Meer, Western Kentucky Univ. (United States)
Matthew A. Raymer, Western Kentucky Univ. (United States)
Shawn L. Wagoner, Western Kentucky Univ. (United States)
Richard L. Hackney, Western Kentucky Univ. (United States)
Joseph M. Beechem, Vanderbilt Univ. (United States)
Enrico Gratton, Univ. of Illinois/Urbana-Champaign (United States)

Published in SPIE Proceedings Vol. 1640:
Time-Resolved Laser Spectroscopy in Biochemistry III
Joseph R. Lakowicz, Editor(s)

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