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Proceedings Paper

Fluorescence lifetime distributions of diphenylhexatrienylpropionyl-labeled phospholipids in atherogenic lipoproteins
Author(s): Andreas Sommer; Roland Gorges; Gerhard M. Kostner; Fritz Paltauf; Albin Hermetter
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Paper Abstract

Fluorescent analogs of phosphatidylcholine (PC) and sphingomyelin (SM) were prepared and incorporated into the surface layer of human low-density lipoprotein (LDL) and lipoprotein (a) [Lp(a)]. Diphenylhexatrienylpropionic acid (DPH) was covalently linked to PC (DPH-PC) and SM (DPH-SM). Fluorescence lifetimes of the labeled lipoproteins were determined by phase and modulation fluorometry. Bimodal Lorentzian distributions for the decay times of DPH-PC and DPH-SM in LDL and Lp(a) were found. Lifetime distribution centers for labeled lipids were very similar except for DPH-PC in Lp(a) which was shifted to longer lifetimes. Thus, PC experiences a less polar environment in Lp(a) than in LDL. The distributional width of DPH-PC in Lp(a) was broader than in LDL. Accordingly, phosphatidylcholine must be localized in a much more homogeneous environment in LDL as compared with Lp(a). On the other hand, no difference in distributional widths was observed for DPH-SM in both lipoproteins, showing that SM organization in Lp(a) is unaffected by apo(a). From the obtained fluorescence data, it is proposed that apoprotein B preferentially associates with phosphatidylcholine.

Paper Details

Date Published: 1 April 1992
PDF: 11 pages
Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); doi: 10.1117/12.58259
Show Author Affiliations
Andreas Sommer, Technische Univ. Graz (Austria)
Roland Gorges, Technische Univ. Graz (Austria)
Gerhard M. Kostner, Univ. Graz (Austria)
Fritz Paltauf, Technische Univ. Graz (Austria)
Albin Hermetter, Technische Univ. Graz (Austria)


Published in SPIE Proceedings Vol. 1640:
Time-Resolved Laser Spectroscopy in Biochemistry III
Joseph R. Lakowicz, Editor(s)

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