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Proceedings Paper

Time-resolved ratio method for multicomponent fluorescence pattern analysis using a fluorescence lifetime imaging system
Author(s): Xue Feng Wang; Takeshi Uchida; Shigeo Minami
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Paper Abstract

Time-resolved ratio method is described for multicomponent pattern analysis by using a fluorescence lifetime imaging system and a ratio method calculation. Nanosecond-level time- resolved fluorescence images of a sample under a pulsed light excitation were detected directly by using a gated microchannel plate (MCP) image intensifier coupled to a CCD camera. Differences in fluorescence lifetimes of components in which each component has similar fluorescence wavelength characteristics were positively utilized to estimate every component spatial distribution. Spatial deconvolution of multicomponent fluorescence data was rapidly carried out from a set of time-resolved, two-dimensional fluorescence data. An application of a multigate time-resolved ratio method for fluorescence pattern analysis was provided. From the results, it is shown that the new method can be successfully used for multicomponent fluorescence pattern analysis at a reasonable signal-to-noise ratio.

Paper Details

Date Published: 1 April 1992
PDF: 7 pages
Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); doi: 10.1117/12.58235
Show Author Affiliations
Xue Feng Wang, Univ. of North Carolina/Chapel Hill (United States)
Takeshi Uchida, Osaka Univ. (Japan)
Shigeo Minami, Osaka Univ. (Japan)


Published in SPIE Proceedings Vol. 1640:
Time-Resolved Laser Spectroscopy in Biochemistry III
Joseph R. Lakowicz, Editor(s)

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