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Proceedings Paper

Infrared imaging of normal and diseased cervical tissue sections
Author(s): Bayden R. Wood; Keith R. Bambery; Lisa M. Miller; Michael Quinn M.D.; Luis Chiriboga; Max Diem; Don McNaughton
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Paper Abstract

Synchrotron FTIR maps, focal plane array and linear array images recorded of 4 μm cervical biopsy sections from the surface epithelium and glandular endometrium are compared in terms of spatial resolution and applicability to the clinical environment. Synchrotron FTIR maps using a 10 μm aperture appear to provide a better spatial resolution capable of discerning single nuclei in the tissue matrix. Unsupervised hierarchical cluster analysis performed on the synchrotron, focal plane array and linear array data in the 1700-1400 cm-1 region show very similar clusters and mean-extracted spectra, demonstrating the robustness of FTIR microscopy and UHCA in the analysis of tissue sections. Maps recorded with the focal plane array using a conventional globar source take one-fortieth of the time but the spatial resolution precludes true single cell analysis in the tissue matrix. The high spatial resolution achieved with the synchrotron shows potential as a gold standard for FTIR diagnosis of cervical samples.

Paper Details

Date Published: 16 February 2005
PDF: 7 pages
Proc. SPIE 5651, Biomedical Applications of Micro- and Nanoengineering II, (16 February 2005); doi: 10.1117/12.582294
Show Author Affiliations
Bayden R. Wood, Monash Univ. (Australia)
Keith R. Bambery, Monash Univ. (Australia)
Lisa M. Miller, Brookhaven National Lab. (United States)
Michael Quinn M.D., Royal Women's Hospital (Australia)
Luis Chiriboga, Bellevue Hosp./NYU Medical Ctr. (United States)
Max Diem, CUNY/Hunter College (United States)
Don McNaughton, Monash Univ. (Australia)

Published in SPIE Proceedings Vol. 5651:
Biomedical Applications of Micro- and Nanoengineering II
Dan V. Nicolau, Editor(s)

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