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Proceedings Paper

Combined photoacoustic and fluorescent quenching studies on organic dyes
Author(s): Cristiano Viappiani; Jeanne Rudzki Small
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Paper Abstract

The development of deconvolution techniques in pulsed-laser, time-resolved photoacoustics has opened the possibility of accurately distinguishing between processes occurring on different time scales, and has given photoacoustics better resolution in determining reaction enthalpies and quantum yields. While fluorescent signals are usually generated by a single de- excitation pathway in the fluorophore, photoacoustic signals usually arise from different sources, such as excited singlet and triplet deactivation, occurring on well-distinguished time scales. The understanding of the effect of quenching on photoacoustic signals therefore requires careful analysis of the data. In this work, a model is developed to describe the effect of fluorescence quenching on photoacoustic signals. The model takes advantage of the time resolution in pulsed-laser photoacoustics. Both static and dynamic quenching are taken into account. Important photophysical parameters (fluorescence and intersystem crossing quantum yields, the bimolecular quenching rate constant, and the volume of the sphere of action) appear in the expressions describing the dependence of photoacoustic signal on quencher concentration. Data from both steady-state fluorescence and time-resolved photoacoustic quenching measurements are analyzed simultaneously using a set of equations containing common parameters. Experimental data on the quenching of organic dyes are presented which support the validity of the model.

Paper Details

Date Published: 1 April 1992
PDF: 10 pages
Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); doi: 10.1117/12.58221
Show Author Affiliations
Cristiano Viappiani, Univ. di Parma (Italy)
Jeanne Rudzki Small, Eastern Washington Univ. (United States)

Published in SPIE Proceedings Vol. 1640:
Time-Resolved Laser Spectroscopy in Biochemistry III
Joseph R. Lakowicz, Editor(s)

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