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Proceedings Paper

Spark source infrared fluorometry
Author(s): Graham Hungerford; David J. S. Birch; Robert E. Imhof
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Paper Abstract

The application of our development of 750 nm excitation from an argon-hydrogen filled spark source to time-resolved fluorescence probe studies of lipid membranes and inverse micelles is reported. The laser dye IR-140 was studied using the single-photon counting technique both in a lipid membrane of L-(alpha) dipalmitoylphosphatidylcholine (DPPC) and in sodium sulfosuccinic acid bis (2-ethylhexyl) ester (AOT) in iso-octane. In DPPC a dramatic change in the fluorescence behavior of IR-140 is observed between the gel and liquid crystalline phases. In inverse micelles of AOT an increase in the intensity of the peak fluorescence emission and a decrease in fluorescence lifetime is noticed on increasing the water content.

Paper Details

Date Published: 1 April 1992
PDF: 9 pages
Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); doi: 10.1117/12.58218
Show Author Affiliations
Graham Hungerford, Univ. of Strathclyde (United Kingdom)
David J. S. Birch, Univ. of Strathclyde (United Kingdom)
Robert E. Imhof, Univ. of Strathclyde (United Kingdom)


Published in SPIE Proceedings Vol. 1640:
Time-Resolved Laser Spectroscopy in Biochemistry III
Joseph R. Lakowicz, Editor(s)

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