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Proceedings Paper

Scanning force microscopy of cells and membrane proteins
Author(s): David Keller; Leda Chang; Ke Luo; Seema Singh; Maxim Yorgancioglu
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Paper Abstract

Recent results on scanning force microscopy of cells and membrane proteins are presented. Whole immune system cells (rat basophil leukemia cells) can be imaged either alive and moving in aqueous medium, frozen, and exposed by freeze fracture (and imaged at -25 degree(s)C), fixed with glutaraldehyde in buffer, or fixed and dried (as if for scanning electron microscopy). Living cells can be stimulated with antigens or drugs and then observed as they move and change shape as a function of time after exposure. In either living or fixed cells it is possible to visualize and map cytoskeletal networks under the cell membrane, and, in living cells, to observe changes in the network with time. Membrane proteins (e.g., the F1 fragment of ATP synthase) can be imaged by simple passive adsorption to freshly cleaved mica. The resolution is about 50 angstroms, which is high enough to identify individual protein molecules, but still too low to distinguish internal structure. Factors which limit resolution and methods that may overcome these limitations are also discussed.

Paper Details

Date Published: 1 May 1992
PDF: 11 pages
Proc. SPIE 1639, Scanning Probe Microscopies, (1 May 1992); doi: 10.1117/12.58194
Show Author Affiliations
David Keller, Univ. of New Mexico (United States)
Leda Chang, Univ. of New Mexico (United States)
Ke Luo, Univ. of New Mexico (United States)
Seema Singh, Univ. of New Mexico (United States)
Maxim Yorgancioglu, Univ. of New Mexico (United States)

Published in SPIE Proceedings Vol. 1639:
Scanning Probe Microscopies
Srinivas Manne, Editor(s)

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