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Proceedings Paper

Digital analysis of fluorescent images as a method of assessing the advancement of cancerous disease
Author(s): Grzegorz Tyc; Zbigniew Palasz; Elzbieta M. Beres-Pawlik; Tomasz Krecicki
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Paper Abstract

Investigations of autofluorescence of cancerous tissues are used in clinical diagnostics. They are only estimative and allow us to define with close approximation the area of cancerously changed tissue. The paper presents a digital analysis of autofluorescent images. The analysis was conducted in the Matlab software environment. Pictures in the RGB range of colors were processed, obtained in similar lighting conditions exciting autofluorescence. As the result of digital processing distributions of intensity were obtained for three basic colors (red, green and blue). The intensity distributions of green and red were shown in three-dimensional space, where the points of the tissue examined were identified on the x y plane, and the intensity bound up with a given color was depicted in the third dimension. On the basis of results obtained this way the intensity ratio of green to red was defined in a selected spot in the examined tissue, and on this basis an attempt at assessing the advancement of cancer was made.

Paper Details

Date Published: 8 September 2004
PDF: 4 pages
Proc. SPIE 5576, Lightguides and their Applications II, (8 September 2004); doi: 10.1117/12.581840
Show Author Affiliations
Grzegorz Tyc, Wroclaw Univ. of Technology (Poland)
Zbigniew Palasz, Wroclaw Univ. of Technology (Poland)
Elzbieta M. Beres-Pawlik, Wroclaw Univ. of Technology (Poland)
Tomasz Krecicki, Wroclaw Medical Univ. (Poland)


Published in SPIE Proceedings Vol. 5576:
Lightguides and their Applications II

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