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Proceedings Paper

Scanning tunneling microscopy of metal-coated biomolecules
Author(s): Ricardo Garcia; Yiqui Jiang; David Dunlap; Carlos J. Bustamante
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Paper Abstract

We present a procedure for imaging metal-coated biomolecules with the scanning tunneling microscope. This procedure is simple, easy to implement and gives reproducible results. The biomolecules are adsorbed on glow discharged mica, then coated with a thin film of platinum- carbon. We have applied this method for visualizing two different kind of biomolecules: planar membranes and DNA molecules. The contrast and lateral resolution of the images are comparable to electron micrographs of the same molecules. The lateral resolution is limited by the grain size of the coating film while the vertical resolution seems to be unaffected for very small coating thickness (less than 3 nm).

Paper Details

Date Published: 1 May 1992
PDF: 9 pages
Proc. SPIE 1639, Scanning Probe Microscopies, (1 May 1992); doi: 10.1117/12.58180
Show Author Affiliations
Ricardo Garcia, Univ. of Oregon (United States)
Yiqui Jiang, Univ. of Oregon (United States)
David Dunlap, Univ. of Oregon (United States)
Carlos J. Bustamante, Univ. of Oregon (United States)

Published in SPIE Proceedings Vol. 1639:
Scanning Probe Microscopies
Srinivas Manne, Editor(s)

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