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Proceedings Paper

Imaging DNA molecules chemically bound to a mica surface
Author(s): Stuart M. Lindsay; Yuri L. Lyubchenko; Anna S. Gall; L. S. Shlyakhtenko; R. E. Harrington
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Paper Abstract

We describe a procedure for imaging DNA molecules using atomic force microscopy (AFM). Double-stranded DNA binds to a mica surface modified by treatment with 3- aminopropyltriethoxysilane to yield AFM images that are stable under repeated scanning. We used this procedure to obtain high quality images of unstained linear DNA molecules and circular DNA molecules. The resolution was limited to tens of nanometers by the profile of the AFM tips.

Paper Details

Date Published: 1 May 1992
PDF: 7 pages
Proc. SPIE 1639, Scanning Probe Microscopies, (1 May 1992); doi: 10.1117/12.58177
Show Author Affiliations
Stuart M. Lindsay, Arizona State Univ. (United States)
Yuri L. Lyubchenko, Arizona State Univ., Univ. of Nevada/Reno (USA), and Institute of Molecular Genetics (Russia)
Anna S. Gall, MicroProbe Corp. (United States)
L. S. Shlyakhtenko, Univ. of Nevada/Reno (USA) and Institute of Molecular Genetics (Russia)
R. E. Harrington, Univ. of Nevada/Reno (United States)

Published in SPIE Proceedings Vol. 1639:
Scanning Probe Microscopies
Srinivas Manne, Editor(s)

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