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Proceedings Paper

Fluorescence correlation spectroscopy considering the effect of photobleaching
Author(s): Yafeng Liu; Tongsheng Chen; Qingming Luo; Shaoqun Zeng
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Paper Abstract

In fluorescence correlation spectroscopy (FCS), the fluorescence intensity decay curve is used to calculate the correlation time, which characterizes the diffusion feature of the molecules. However, photobleaching, an unavoidable phenomenon that happened during fluorescence excitation, would result in fluorescence decay, and thus cause error in the calculation of the correlation time and the diffusion coefficient. To compensate this effect, a probability factor is introduced. This factor describes the probability at which a molecule is photobleached by the excitation laser during fluorescence excitation. By selecting the right value of the photobleaching factor, the effect of photobleaching on the FCS measurement can be removed.

Paper Details

Date Published: 18 January 2005
PDF: 7 pages
Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); doi: 10.1117/12.580645
Show Author Affiliations
Yafeng Liu, Huazhong Univ. of Science and Technology (China)
Tongsheng Chen, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 5630:
Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II
Britton Chance; Mingzhe Chen; Arthur E. T. Chiou; Qingming Luo, Editor(s)

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