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Proceedings Paper

Time domain transmission line dielectric probe for detection of biomolecular interactions
Author(s): Qin Chen; Daniel B. Roitman; Andre Knoesen
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Paper Abstract

A probe was developed to detect biomolecular binding events on a substrate in the microwave regime in real time and without labels. The probe consists of a coplanar transmission line fabricated on a glass slide that can detect dielectric changes in close proximity of the substrate-liquid interface. The probe behavior was evaluated by adsorbing polyelectrolyte monolayers of alternating charge. Biomolecular detection was demonstrated by immobilizing protein A on the glass surface and detecting rabbit IgG molecules in a flow channel. The sensitivity of the probe was conservatively estimated to be ~100 pg/mm2.

Paper Details

Date Published: 8 December 2004
PDF: 7 pages
Proc. SPIE 5591, Lab-on-a-Chip: Platforms, Devices, and Applications, (8 December 2004); doi: 10.1117/12.580138
Show Author Affiliations
Qin Chen, Univ. of California/Davis (United States)
Daniel B. Roitman, Agilent Technologies (United States)
Andre Knoesen, Univ. of California/Davis (United States)

Published in SPIE Proceedings Vol. 5591:
Lab-on-a-Chip: Platforms, Devices, and Applications
Linda A. Smith; Daniel Sobek, Editor(s)

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