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Proceedings Paper

Effect of serotonin on the expression of antigens and DNA levels in Yersinia pestis cells with different plasmid content
Author(s): Svetlana N. Klueva; Vladimir N. Korsukov; Tatyana N. Schukovskaya; Alexander L. Kravtsov
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Paper Abstract

Using flow cytometry (FCM) the influence of exogenous serotonin on culture growth, DNA content and fluorescence intensity of cells binding FITC-labelled plague polyclonal immunoglobulins was studied in Yersinia pestis EV (pFra+, pCad+, pPst+), Yersinia pestis KM218 (pFra-, pCad-, pPst-), Yersinia pestis KM 216 (pFra-, pCad-, pPst+). The results have been obtained by FCM showed serotonin accelerated Yersinia pestis EV (pFra+, pCad+, pPst+), Yersinia pestis KM218 (pFra-, pCad-, pPst-) culture growth during cultivation in Hottinger broth pH 7.2 at 28°C at concentration of 10-5 M. The presence of 10-5 M serotonin in nutrient broth could modulate DNA content in 37°C growing population of plague microbe independently of their plasmid content. Serotonin have been an impact on the distribution pattern of the cells according to their phenotypical characteristics, which was reflected in the levels of population heterogeneity in the intensity of specific immunofluorescence determined by FMC.

Paper Details

Date Published: 20 August 2004
PDF: 8 pages
Proc. SPIE 5474, Saratov Fall Meeting 2003: Optical Technologies in Biophysics and Medicine V, (20 August 2004); doi: 10.1117/12.579011
Show Author Affiliations
Svetlana N. Klueva, Russian Anti-Plague Research Institute (Russia)
Vladimir N. Korsukov, Russian Anti-Plague Research Institute (Russia)
Tatyana N. Schukovskaya, Russian Anti-Plague Research Institute (Russia)
Alexander L. Kravtsov, Russian Anti-Plague Research Institute (Russia)


Published in SPIE Proceedings Vol. 5474:
Saratov Fall Meeting 2003: Optical Technologies in Biophysics and Medicine V

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