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Proceedings Paper

On the role of adenylate cyclase, tyrosine kinase, and tyrosine phosphatase in the response of nerve and glial cells to photodynamic impact
Author(s): Mikhail S. Kolosov; D. E. Bragin; Olga Yu. Dergacheva; O. Vanzha; L. Oparina; Anatoly B. Uzdensky
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Paper Abstract

The role of different intercellular signaling pathways involving adenylate cyclase (AC), receptor tyrosine kinase (RTK), tyrosine and serine/threonine protein phosphatases (PTP or PP, respectively) in the response of crayfish mechanoreceptor neuron (MRN) and surrounding glial cells to photodynamic effect of aluminum phthalocyanine Photosens have been studied. AC inhibition by MDL-12330A decreased neuron lifetime, whereas AC activation by forskolin increase it. Thus, increase in cAMP produced by activated AC protects SRN against photodynamic inactivation. Similarly, RTK inhibition by genistein decreased neuron lifetime, while inhibition of PTP or PP that remove phosphate groups from proteins, prolonged neuronal activity. AC inhibition reduced photoinduced damage of the plasma membrane, and, therefore, necrosis in neuronal and glial cells. RTK inhibition protected only neurons against PDT-induced membrane permeabilization while glial cells became lesser permeable under ortovanadate-mediated PTP inhibition. AC activation also prevented PDT-induced apoptosis in glial cells. PP inhibition enhanced apoptotic processes in photosensitized glial cells. Therefore, both intercellular signaling pathways involving AC and TRK are involved in the maintenance of neuronal activity, integrity of the neuronal and glial plasma membranes and in apoptotic processes in glia under photosensitization.

Paper Details

Date Published: 20 August 2004
PDF: 9 pages
Proc. SPIE 5474, Saratov Fall Meeting 2003: Optical Technologies in Biophysics and Medicine V, (20 August 2004); doi: 10.1117/12.579009
Show Author Affiliations
Mikhail S. Kolosov, Rostov State Univ. (Russia)
D. E. Bragin, Rostov State Univ. (Russia)
Olga Yu. Dergacheva, Rostov State Univ. (Russia)
O. Vanzha, Rostov State Univ. (Russia)
L. Oparina, Rostov State Univ. (Russia)
Anatoly B. Uzdensky, Rostov State Univ. (Russia)


Published in SPIE Proceedings Vol. 5474:
Saratov Fall Meeting 2003: Optical Technologies in Biophysics and Medicine V

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