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Proceedings Paper

Optical control of a rhodopsin-based switch
Author(s): Ben Ovryn; Xiang Li; Hillel Chiel; Stefan Herlitze
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Paper Abstract

A preliminary result supports the feasibility of using visible light to modulate the membrane potential of a cell. Human embryonic kidney cells (HEK293) were transfected with vertebrate rhodopsin and a gradient inward rectifying potassium (GIRK) channel. Whole cell patch clamp recordings of HEK293 cells exposed to 9-cis retinal showed that illumination increases the potassium current compared with recordings obtained in the dark. When combined with a rapid scanning device, this approach has the potential to control the activity of many neurons.

Paper Details

Date Published: 13 July 2004
PDF: 11 pages
Proc. SPIE 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI, (13 July 2004); doi: 10.1117/12.578942
Show Author Affiliations
Ben Ovryn, Albert Einstein College of Medicine (United States)
Xiang Li, Case Western Reserve Univ. (United States)
Hillel Chiel, Case Western Reserve Univ. (United States)
Stefan Herlitze, Case Western Reserve Univ. (United States)


Published in SPIE Proceedings Vol. 5324:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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