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Proceedings Paper

Monitoring of small lymphatics function under different impact on animal model by integrated optical imaging
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Paper Abstract

The digital transmission microscopy is very informative, noninvasive for vessels, simple and available method for studying and measuring lymph microvessels function in vivo. Rat mesentery can use as promising animal model of lymph microvessels in vivo. Such imaging system allowed visualizing the entire lymphangion (with input and output valves), its wall, lymphatic valves, lymph flow as well as single cells in flow; obtaining anew basic information on lymph microcirculation and quantitative data on lymphatic function including indexes of phasic contractions and valve function, the quantitative parameters of lymph-flow velocity. Rat mesentery is good model to create different types of lymphedemas in acute and chronic experiments. The obtained data revealed that significant edema started immediately after lymph node dissection in one-half of cases and was accompanied by lymphatic disturbances. The greatest degree of edema was found after 1 week. After 4 weeks, the degree of edema sometimes decreased, but functional lymphatic disturbances progressed. Nicotine had significant direct dose-dependent effect on microlymphatic function at the acute local application, but the same dose of this drug was not effect on microcirculation in chronic intoxication. Despite yielding interesting data, transmittance microscopy had some limitations when applied to microcirculation studies. The problems could be solved at the application of integrated measuring technique.

Paper Details

Date Published: 20 August 2004
PDF: 11 pages
Proc. SPIE 5474, Saratov Fall Meeting 2003: Optical Technologies in Biophysics and Medicine V, (20 August 2004); doi: 10.1117/12.578915
Show Author Affiliations
Ekaterina I. Galanzha, Saratov State Univ. (Russia)
Valery V. Tuchin, Saratov State Univ. (Russia)
Parimal Chowdhury, Univ. of Arkansas for Medical Sciences (United States)
Vladimir P. Zharov, Univ. of Arkansas for Medical Sciences (United States)


Published in SPIE Proceedings Vol. 5474:
Saratov Fall Meeting 2003: Optical Technologies in Biophysics and Medicine V
Valery V. Tuchin, Editor(s)

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