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Proceedings Paper

AlGaN-based deep-UV LEDs for fluorescence sensing
Author(s): Pranciskus Vitta; Natalija Kurilcik; Algirdas Novickovas; Saulius Jursenas; Henrikas Calkauskas; Arturas Zukauskas; Remis Gaska
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Paper Abstract

Recent progress in wide-bandgap semiconductor optoelectronics resulted in an appearance of deep-UV light-emitting diodes (LEDs), which can be used for fluorescence excitation in a variety of chemical and biological compounds. We used two generations of AlGaN-based UVTOP series deep ultraviolet LEDs developed by Sensor Electronic Technology, Inc. The peak wavelength of these fully packaged devices is 340 nm and 280 nm, line width at half maximum approximately 10 nm, wall-plug efficiency up to 0.9% and output power in the milliwatt range. The second-generation emitters are shown to have an extremely low level of unwanted long-wavelength emission what is important for fluorescence measurements. The UV LEDs were tested for fluorescence excitation in standard fluorophores (organic dyes), autofluorescent biological compounds (riboflavin, NADH, tryptophan, and tyrosine) and medical specimens (fluid secreted by prostate gland). Fluorescence lifetime measurements in the frequency domain were demonstrated using UVTOP-340 and -280 devices. The output of the LEDs was modulated at frequencies up to 200 MHz by high-frequency current drivers and the phase angle of the fluorescence signal was resolved using a radio-frequency lock-in amplifier. Nanosecond-scaled measurements of fluorescence lifetimes, which are the “fingerprints” of chemical and biological compounds, were demonstrated.

Paper Details

Date Published: 29 December 2004
PDF: 12 pages
Proc. SPIE 5617, Optically Based Biological and Chemical Sensing for Defence, (29 December 2004); doi: 10.1117/12.578684
Show Author Affiliations
Pranciskus Vitta, Vilnius Univ. (Lithuania)
Natalija Kurilcik, Vilnius Univ. (Lithuania)
Algirdas Novickovas, Vilnius Univ. (Lithuania)
Saulius Jursenas, Vilnius Univ. (Lithuania)
Henrikas Calkauskas, Vilnius Univ. (Lithuania)
Arturas Zukauskas, Vilnius Univ. (Lithuania)
Remis Gaska, Sensor Electronic Technology, Inc. (United States)

Published in SPIE Proceedings Vol. 5617:
Optically Based Biological and Chemical Sensing for Defence
John C. Carrano; Arturas Zukauskas, Editor(s)

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