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Proceedings Paper

Raman spectroscopic measurements of beta-carotene and lycopene in human skin
Author(s): M. E. Darvin; I. Gerzonde; S. Ey; Nikolai N. Brandt; Hansjoerg Albrecht; Sergei A. Gonchukov; Wolfram Sterry; Juergen Lademann
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Paper Abstract

The antioxidant β-carotene and lycopene substances were detected non-invasively, in vivo in human skin using resonance Raman spectroscopy. Both substances were detected simultaneously. To distinguish between the substances, the Raman signals were excited at 488 nm and 514,5 nm simultaneously using a multilane Ar+ laser. The application of a fiber based optical imaging system allowed the detection of β-carotene and lycopene on any skin area. The disturbance of the measurements because of non-homogeneous skin pigmentation was avoided by using a measuring area of 28 mm2. The minimum power density for registration of the Raman signals and their optimum relation was determined. The Raman spectroscopic method is well suited for the evaluation of the efficacy of topically or systematically applied amounts of β-carotene and lycopene.

Paper Details

Date Published: 20 August 2004
PDF: 5 pages
Proc. SPIE 5474, Saratov Fall Meeting 2003: Optical Technologies in Biophysics and Medicine V, (20 August 2004); doi: 10.1117/12.578308
Show Author Affiliations
M. E. Darvin, Charite Univ. Hospital (Germany)
I. Gerzonde, Laser- und Medizin-Technologie GmbH (Germany)
S. Ey, Laser- und Medizin-Technologie GmbH (Germany)
Nikolai N. Brandt, Moscow State Univ. (Russia)
Hansjoerg Albrecht, Laser- und Medizin-Technologie GmbH (Germany)
Sergei A. Gonchukov, Moscow Engineering Physics Institute (Russia)
Wolfram Sterry, Charite Univ. Hospital (Germany)
Juergen Lademann, Charite Univ. Hospital (Germany)

Published in SPIE Proceedings Vol. 5474:
Saratov Fall Meeting 2003: Optical Technologies in Biophysics and Medicine V
Valery V. Tuchin, Editor(s)

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