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Proceedings Paper

Kinetics of camptothecin deactivation determined by time evolution of fluorescence and absorption spectra
Author(s): Blanka Ziomkowska; Stefan Kruszewski; Ryszard Siuda; Michal Cyrankiewicz
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Paper Abstract

Fluorescence and absorption spectra of camptothecin -- anticancer alkaloid, are analyzed in this paper. Camptothecin exists in two forms: lactone and carboxylate. Only lactone form is biologically active. In physiological fluids at pH 7.4 lactone form hydrolyses and converts into carboxylate form. There are some differences in shapes between fluorescence and absorption spectra of lactone and carboxylate form. In the deactivation process fluorescence and absorption spectra evaluate. Thanks to the factor analysis of series of spectra recorded during the process of hydrolysis one can follow the concentration of both forms in the solution. Described method could be useful in determining of kinetics of deactivation process and competitive with the HPLC method.

Paper Details

Date Published: 7 July 2004
PDF: 6 pages
Proc. SPIE 5505, Optical Methods, Sensors, Image Processing, and Visualization in Medicine, (7 July 2004); doi: 10.1117/12.577945
Show Author Affiliations
Blanka Ziomkowska, Ludwik Rydygier Medical Univ. (Poland)
Univ. of Technology and Agriculture (Poland)
Stefan Kruszewski, Ludwik Rydygier Medical Univ. (Poland)
Univ. of Technology and Agriculture (Poland)
Ryszard Siuda, Univ. of Technology and Agriculture (Poland)
Michal Cyrankiewicz, Ludwik Rydygier Medical Univ. (Poland)
Univ. of Technology and Agriculture (Poland)


Published in SPIE Proceedings Vol. 5505:
Optical Methods, Sensors, Image Processing, and Visualization in Medicine
Antoni Nowakowski; Bogdan B. Kosmowski, Editor(s)

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