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Proceedings Paper

Factor analysis of excitation fluorescence spectra of camptothecin and its analogue: DB-67
Author(s): Stefan Kruszewski; Blanka Ziomkowska; Ryszard Siuda; Michal Cyrankiewicz
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Paper Abstract

The fluorescence excitation spectra of camptothecins -- alkaloids which have anticancer properties, are analyzed in this paper. Camptothecins exist in two forms -- biologically active lactone form and inactive carboxylate form. In fluids of pH 7,4 lactone forms hydrolyze and convert into carboxylate forms. There are some differences in shapes between fluorescence excitation spectra of lactone and carboxylate forms. One can observe evolution of fluorescence spectra during hydrolysis of camptothecins. Using principal component analysis and factor analysis to the set of fluorescence spectra recorded during process of hydrolysis of camptothecins one can determine the temporary concentration of lactone and carboxylate forms in solutions of pH 7,4 and obtain kinetics of deactivation of this compounds.

Paper Details

Date Published: 7 July 2004
PDF: 6 pages
Proc. SPIE 5505, Optical Methods, Sensors, Image Processing, and Visualization in Medicine, (7 July 2004); doi: 10.1117/12.577611
Show Author Affiliations
Stefan Kruszewski, Ludwik Rydygier Medical Univ. (Poland)
Univ. of Technology and Agriculture (Poland)
Blanka Ziomkowska, Ludwik Rydygier Medical Univ. (Poland)
Univ. of Technology and Agriculture (Poland)
Ryszard Siuda, Univ. of Technology and Agriculture (Poland)
Michal Cyrankiewicz, Ludwik Rydygier Medical Univ. (Poland)
Univ. of Technology and Agriculture (Poland)


Published in SPIE Proceedings Vol. 5505:
Optical Methods, Sensors, Image Processing, and Visualization in Medicine
Antoni Nowakowski; Bogdan B. Kosmowski, Editor(s)

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