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Proceedings Paper

Confocal microscopy for the life sciences
Author(s): G. J. Brakenhoff; H. T. M. van der Voort; Koen Visscher
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Paper Abstract

In the confocal scanning light microscope a specific volume is sampled during the imaging process. The physical process is explained, together with how the size of the pinholes used affect the actual size of this volume. The thus produced 3-D imaging is of high quality but subject to a number of limitations. A novel (bilateral scanning) arrangement is presented that may relieve some of these. In this approach, a double-sided scanning mirror element and a charge coupled device (CCD for image collection) are used.

Paper Details

Date Published: 1 March 1992
PDF: 6 pages
Proc. SPIE 1524, Bioptics: Optics in Biomedicine and Environmental Sciences, (1 March 1992); doi: 10.1117/12.57721
Show Author Affiliations
G. J. Brakenhoff, Univ. of Amsterdam (Netherlands)
H. T. M. van der Voort, Univ. of Amsterdam (Netherlands)
Koen Visscher, Univ. of Amsterdam (Netherlands)


Published in SPIE Proceedings Vol. 1524:
Bioptics: Optics in Biomedicine and Environmental Sciences

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