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Proceedings Paper

Attenuation of excitation and emission path of epifluorescence microscope
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Paper Abstract

Spectral properties of excitation and emission path of epifluorescence microscope at object and image planes has been determined and analyzed for two of filter sets. Attenuation of excitation path at object plane and attenuation of emission path at image plane were proved to be useful for this purpose. Calculations were carried out for epifluorescence filters made for FITC (fluorescein) type fluorophores (494ex/518em).

Paper Details

Date Published: 26 August 2004
PDF: 7 pages
Proc. SPIE 5566, Optical Security and Safety, (26 August 2004); doi: 10.1117/12.577168
Show Author Affiliations
Grzegorz Jerzy Kopec, Institute of Applied Optics (Poland)


Published in SPIE Proceedings Vol. 5566:
Optical Security and Safety
Zbigniew Jaroszewicz; Ewa Powichrowska; Mariusz Szyjer, Editor(s)

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