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Proceedings Paper

Laser-induced fluorescence imaging system for protein separations in microfluidic devices
Author(s): Champak Das; Alexander Stoyanov; Carl Fredrickson; Roger Tran-Son-Tay; Zhonghui Hugh Fan
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Paper Abstract

This paper describes a laser-induced fluorescence (LIF) detection system for imaging proteins separated in a microfluidic device. The diameter of a laser beam is first increased through a beam expander, and subsequently focused into a line using a cylindrical lens. The resultant laser line is used to image an entire capillary or channel in which protein separation took place. The fluorescence emission is collected with a cooled, scientific grade charge-coupled device (CCD) camera. The detection limit was determined using a series of concentrations of fluorescein solutions. The temporal and spatial effects of photobleaching from laser irradiation were analyzed and the parameters to reduce the effect of photobleaching are discussed. We used the imaging system to demonstrate rapid analysis of proteins using isoelectric focusing.

Paper Details

Date Published: 8 December 2004
PDF: 8 pages
Proc. SPIE 5591, Lab-on-a-Chip: Platforms, Devices, and Applications, (8 December 2004); doi: 10.1117/12.576887
Show Author Affiliations
Champak Das, Univ. of Florida (United States)
Alexander Stoyanov, Univ. of Florida (United States)
Carl Fredrickson, Univ. of Florida (United States)
Roger Tran-Son-Tay, Univ. of Florida (United States)
Zhonghui Hugh Fan, Univ. of Florida (United States)

Published in SPIE Proceedings Vol. 5591:
Lab-on-a-Chip: Platforms, Devices, and Applications
Linda A. Smith; Daniel Sobek, Editor(s)

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