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Proceedings Paper

Application of chemiluminescence with FCLA in photodynamic therapy
Author(s): Yanfang Qin; Da Xing; Xueyun Zhong; Jing Zhou; Shiming Luo; Qun Chen
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Paper Abstract

In photodynamic therapy (PDT), a target tissue with pre-administered photosensitizer is exposed to laser light. The photochemical process produces reaction oxygen species (ROS), such as singlet oxygen and superoxide, and leads to ultimate cell death. A direct monitoring of ROS production during PDT, thus, may provide important information in both basic science and clinical practice. A cypridina luciferin analogue (FCLA) is a chemiluminescence (CL) probe that selectively detects singlet oxygen and superoxide. In this study, FCLA was used as an optical reporter of ROS produced by photosensitization reaction of Photofrin in Hanks solution and the CL was measured by a photomultiplier system operated at single photon counting mode. By varying the amount of PDT dosage (photosensitizer dose, light irradiation fluence rate) and the amount of FCLA, the intensity of CL were investigated. The results showed the FCLA concentration affects the ratio of the signal to background CL. The decay time of the photosensitized CL was approximately 10 sec., after the excitation source was turned off. In addition, the intensity of the CL-FCLA increased with increasing concentration of Photofrin and fluence rate. The work supported the potential application of FCLA-chemiluminescence probe as a dosimetric tool for PDT.

Paper Details

Date Published: 18 January 2005
PDF: 6 pages
Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); doi: 10.1117/12.575856
Show Author Affiliations
Yanfang Qin, South China Normal Univ. (China)
Jinan Univ. (China)
Da Xing, South China Normal Univ. (China)
Xueyun Zhong, Jinan Univ. (China)
Jing Zhou, South China Normal Univ. (China)
Shiming Luo, South China Normal Univ. (China)
Qun Chen, South China Normal Univ. (China)
HealthONE Alliance (United States)


Published in SPIE Proceedings Vol. 5630:
Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II
Britton Chance; Mingzhe Chen; Arthur E. T. Chiou; Qingming Luo, Editor(s)

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