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Proceedings Paper

Detection of immune reaction by dynamic enhanced vibrational spectroscopy of molecules in near field
Author(s): Haiying Zhao; Xiaoming Dou
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Paper Abstract

This paper reports the near-infrared (NIR) SERS detection of immune reaction on gold colloid particles without bound/Free (B/F) antigen separation. Antibodies or the immune complex are adsorbed on gold colloid particles. Free antigen cannot be adsorbed on the gold colloid particles due to the surface of the gold colloid particles is blocked by bovine serum albumin. Because of the electromagnetic field generated by surface plasmon polariton enhancement, the antibody at 10-8 M on the gold colloid particles shows intense SERS signals. The same system at 10-10 M does not give any SERS signal, but Raman bands of antibody again appear upon the formation of immunoglobulin (IgG)-anti-IgG complexes on the gold colloid particles. Free and bound antigen molecules do not yield significant SERS signals since they cannot be adsorbed directly on the gold surface. In this way, one could detect the immune complex on the gold colloid particles by NIR SERS spectroscopy without any B/F antigen separation.

Paper Details

Date Published: 18 January 2005
PDF: 6 pages
Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); doi: 10.1117/12.575753
Show Author Affiliations
Haiying Zhao, Shanghai Jiaotong Univ. (China)
Xiaoming Dou, Shanghai Jiaotong Univ. (China)

Published in SPIE Proceedings Vol. 5630:
Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II
Britton Chance; Mingzhe Chen; Arthur E. T. Chiou; Qingming Luo, Editor(s)

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