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Proceedings Paper

New results in human eye laser diagnostics
Author(s): L. P. Shubochkin; Valery V. Tuchin
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Paper Abstract

Different laser spectroscopy methods are applicable in ophthalmology for diseases diagnostics and biotissues state monitoring. Both groups of micro- and macrodiagnostics methods, especially laser Raman, resonance Raman, surface-enhanced Raman (SERS), Raman microprobe, fluorescence, Raman/fluorescence, infrared Fourier- transformation absorption method (FT-IR) and the method with the use of total reflection prism (ATR/FT-IR), FT-Raman , elastic and quasi-elastic light scattering spectroscopy have good possibilities in human and mammalia eye tissues diagnostics.''2 Microdiagnostics is realyzed with the help of all kinds of Raman, absorption and fluorescence spectroscopy and allows to study macromolecular structure and biochemical changes in eye tissues. These methods are very promising in analyzing of cataract mechanism formation, but not all of them can be used in early human cataract diagnostics, as far as they require comparatively high levels of laser radiation power. For example, modern means of Raman spectroscopy (computer controlled optical multichannel analyzer) in early cataract diagnostics need the crystalline lens has to be radiated by Ar laser with the power of 1 mW during is (Refs. 2,4). Macrodiagnostics of biotissues exploit such methods as elastic and quasi-elastic light scattering, interferometry, polarimetry and specle optics. These methods give information about form and dimensions of individual scattering particale of optically inhomogeneous media, distribution function of particales dimensions, optical constants, static characteristics of particles spatial location and presence of anisotropy. The investigations carried out for different eye. tissues demonstrate the possibility of use elastic light scattering techniques to diseases diagnostics and biotissues state monitoring in ophthalmology. Elastic scattering, interferometry and polarimetry methods require low levels of laser radiation power and comparatively simple installation for their realization. That's why they are very suitable for in vivo human eye diagnostics. In this paper laser light diagnostics methods and aapparatus for their realization are considered. Elastic laser light scattering, interferometry and polarimetry methods are used. The devices and techniques for the in vivo investigations of light scattering of human eye biotissues are described.

Paper Details

Date Published: 1 May 1991
PDF: 12 pages
Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); doi: 10.1117/12.57356
Show Author Affiliations
L. P. Shubochkin, Institute of Radio Engineering and Electronics (Russia)
Valery V. Tuchin, Saratov State Univ. (Russia)

Published in SPIE Proceedings Vol. 1403:
Laser Applications in Life Sciences
Sergei A. Akhmanov; Marina Yu. Poroshina, Editor(s)

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