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Proceedings Paper

Improvement of hybridization signals of colorimetric gene detection based on porous polypropylene membranes
Author(s): Libo Nie; Ancun Zhou; Hong Chen; Quanguo He; Nongyue He
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Paper Abstract

Porous polypropylene (PP) membranes were modified by the plasma treatment in order to graft amino functional group (-NH2) onto the membrane surface. Oligonucleotides were in situ synthesized on the aminated polypropylene support. Gold nanoparticle labeled DNAs were bybridized to the synthesized oligonucleotide array. The membranes were exposed to the Silver Enhance Solution for singla amplification. The Hybridization signals of amino plasma-grafted polypropylene membranes were stronger than the commercial polyacrylamide modified polyproplylene membranes that load 0.07 μmol/cm2 free primary amino functions. Complementary and mismatched sequences were clearly distinguished. The diameter of nanogold particles and the concentration of thiol DNA modified gold nanoparticles were investigated to improve the hybridization signals. Bigger nanoparticle diameter, as well as higher concentration of thiol DNA modified gold nanoparticles lead to stronger hybridization signals.

Paper Details

Date Published: 29 December 2004
PDF: 5 pages
Proc. SPIE 5593, Nanosensing: Materials and Devices, (29 December 2004); doi: 10.1117/12.573537
Show Author Affiliations
Libo Nie, Zhuzhou Institute of Technology (China)
Ancun Zhou, College of Hunan Traditional Chinese Medicine (China)
Hong Chen, Zhuzhou Institute of Technology (China)
Central South Forestry Univ. (China)
Quanguo He, Zhuzhou Institute of Technology (China)
Nongyue He, Zhuzhou Institute of Technology (China)
Zhongshan Polytechnic (China)

Published in SPIE Proceedings Vol. 5593:
Nanosensing: Materials and Devices
M. Saif Islam; Achyut K. Dutta, Editor(s)

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