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Proceedings Paper

In situ synthesis of oligonucleotides on plasma-modified PTFE for fabricating DNA chips
Author(s): Nongyue He; Jianxin Tang; Libo Nie; Hong Chen
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Paper Abstract

Oligonucleotide probe arrays were in-situ synthesized on the H2/N2 plasma modified poly(terafluoroethylene) (PTFE) surface via micro-fluidic channels connected with an automated synthesizer. A contact angle measurement of water droplets was used to ascertain the hydrophilicity of the surface. X-ray photoelectron spectroscopy (XPS) analysis confirmed the presence of polar amino groups on the surface. Ultra-violet (UV) spectrum analysis indicated that the surface showed a coupling efficiency higher than 98% for in situ synthesis of oligonucleotides arrays. The probe array specificity was discriminated by hybridizing with fluorescent target sequencyes. Oligonucleotide probe arrays on modified PTFE surface showed high stability and durability after repetitive denaturing and hybridizing. The results implied the plasma modified PTFE surface was extremely stable, performed well in DNA hybridization assays and could service as a good substrate for high-density oligonucleotide array synthesis.

Paper Details

Date Published: 29 December 2004
PDF: 8 pages
Proc. SPIE 5593, Nanosensing: Materials and Devices, (29 December 2004); doi: 10.1117/12.573464
Show Author Affiliations
Nongyue He, Zhuzhou Institute of Technology (China)
Zhongshan Huoju Polytechnic College (China)
Jianxin Tang, Zhuzhou Institute of Technology (China)
Libo Nie, Zhuzhou Institute of Technology (China)
Hong Chen, Zhuzhou Institute of Technology (China)
Central South Forestry Univ. (China)
Zhongshan Huoju Polytechnic College (China)

Published in SPIE Proceedings Vol. 5593:
Nanosensing: Materials and Devices
M. Saif Islam; Achyut K. Dutta, Editor(s)

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