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Proceedings Paper

Sequence dependence of the length of the B to Z junctions in DNA
Author(s): Warner L. Peticolas; Zhongy Dai; G. A. Thomas
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Paper Abstract

The Raman spectra of several duplexes of self-complementary oligodeoxynucleotides such as d[TTCGCGCGCGAA] have been taken under various conditions of salt concentration and temperature. It is shown how to determine the number of CG base pairs in the Z form and how many in the junction between the B and Z forms in these oligomers. The intensities of the guanine-backbone bands at 680 cm'' and 625 cm'' are used. As noted by M. Tsuboi Y. Nishimura G. J. Thomas Jr. and their coworkers the former frequency is the marker band for the B form or the disordered form while the latter frequency is the marker band for the Z form. The marker bands of the AT base pairs previously determined by Taillandier and Liquier can be used to rule out the occurrence of the Z form in the AT portion of the duplex. From these measurements the structure of the double helical duplex in concentrated salt solutions can be described as having four of the six interior CG base pairs in Z form flanked by two CG base pairs in the junction and the two AT base pairs in a short B form segment. Similar measurements on a large number of oligomers shows that both single B-Z and double B-Z-B junctions may be present both in the solution and in single crystals.

Paper Details

Date Published: 1 May 1991
PDF: 5 pages
Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); doi: 10.1117/12.57319
Show Author Affiliations
Warner L. Peticolas, Univ. of Oregon (United States)
Zhongy Dai, Univ. of Oregon (United States)
G. A. Thomas, Univ. of Oregon (United States)


Published in SPIE Proceedings Vol. 1403:
Laser Applications in Life Sciences
Sergei A. Akhmanov; Marina Yu. Poroshina, Editor(s)

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