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Proceedings Paper

Drug-target interactions on a single living cell: an approach by optical microspectroscopy
Author(s): Michel Manfait; Hamid Morjani; Jean-Marc Millot; Vincent Debal; Jean-Francois Angiboust; Igor R. Nabiev
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Paper Abstract

Optical microspectroscopic techniques - both powerful and nondestructive - are particularly suited to the study within a single living cell of molecular interactions between drugs (anticancer agents or xenobiotics) and their pharmacological targets. These techniques include vibrational spectroscopy : Raman spectroscopy (conventional resonance SERS) and Fourier transform infrared microspectroscopy together with fluorescence microspectroscopy. Applications are given Ci) in the subcompartmental quantitative analysis of anthracyclines and ymca-alkaloids and (ii) in the study within the cell of the molecular interactions of these compounds with their target molecules. Among these techniques laser microspectrofluorometry is easy to operate and allows the study of intracellular drug pharmacokinetics related to biological characteristics such as cell resistance or differentiation.

Paper Details

Date Published: 1 May 1991
PDF: 13 pages
Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); doi: 10.1117/12.57316
Show Author Affiliations
Michel Manfait, Univ. de Reims (France)
Hamid Morjani, Univ. de Reims (France)
Jean-Marc Millot, Univ. de Reims (France)
Vincent Debal, Univ. de Reims (France)
Jean-Francois Angiboust, Univ. de Reims (France)
Igor R. Nabiev, Univ. de Reims (France) and Shemyakin Institute of Bio-organic Chemistry (Russia)

Published in SPIE Proceedings Vol. 1403:
Laser Applications in Life Sciences
Sergei A. Akhmanov; Marina Yu. Poroshina, Editor(s)

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