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Proceedings Paper

DNA photoproducts formed using high-intensity 532-nm laser radiation
Author(s): Irene E. Kochevar; Yaron Hefetz; D. A. Dunn; T. F. Deutsch; Lisa A. Buckley; Franz Hillenkamp
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Paper Abstract

Cyclobutylpyrimidine dimers were detected in double-stranded plasmid DNA after exposure to 532-nm 28-ps pulses at intensities (1-8 GW/cm2) below the threshold for optical breakdown. Formation of these photoproducts was attributed to simultaneous two-photon absorption by nucleotides in DNA because the rate of dimer formation was dependent on the square of the intensity and because an equivalent number of photons at 514 nm delivered at low intensity did not produce dimers. The average two-photon cross-section at 532 rim for absorption by a nucleotide was found to be 0. 50 ( 0. 02) x l052 cm4sec'' photon* Intensities of 532-rim radiation above the threshold for optical breakdown caused DNA strand breaks by radical and mechanical mechanisms. 1 .

Paper Details

Date Published: 1 May 1991
PDF: 8 pages
Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); doi: 10.1117/12.57311
Show Author Affiliations
Irene E. Kochevar, Harvard Medical School and Massachusetts General Hospital (United States)
Yaron Hefetz, Harvard Medical School and Massachusetts General Hospital (United States)
D. A. Dunn, Harvard Medical School and Massachusetts General Hospital (United States)
T. F. Deutsch, Harvard Medical School and Massachusetts General Hospital (United States)
Lisa A. Buckley, Harvard Medical School and Massachusetts General Hospital (United States)
Franz Hillenkamp, Univ. Muenster (Germany)


Published in SPIE Proceedings Vol. 1403:
Laser Applications in Life Sciences
Sergei A. Akhmanov; Marina Yu. Poroshina, Editor(s)

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