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Proceedings Paper

Study on molecular interactions of bovine serum albumin in electrolyte solutions by dynamic light scattering
Author(s): Junfeng Li; Da Xing; Shaoxin Li
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Paper Abstract

The concentration dependence of the diffusion coefficient of particles suspended in solution depends primarily on the occupied volume fraction and on repulsive and attractive forces. This dependency is expressed by the interaction parameter λ. In the present work we have measured the diffusion coefficient of Bovine Serum Albumin at different ionic strength and determined the interaction parameter. The results indicate: the value of λ is positive at low ionic strength and the interaction between proteins is repulsive; however, with increasing ionic strength the value of λ becomes negative and the interaction is attractive, and when ionic strength is bigger than 0.50M aggregation occurs. The dependence of the interaction on ionic strength is interpreted using DLVO theory for interactions of two hard spheres: with increasing ionic strength, the repulsive electrostatic interaction is screened and Van der Waals forces become dominant. According to the correlation of λ with ionic strength, the protein parameters are regressed: the protein net charge ZP= -9.0e, Hamaker constant HA= 2.8kBT. This work indicates the technique of dynamic light scattering can be used effectively to study protein molecular interactions.

Paper Details

Date Published: 18 January 2005
PDF: 9 pages
Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); doi: 10.1117/12.572820
Show Author Affiliations
Junfeng Li, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Shaoxin Li, South China Normal Univ. (China)


Published in SPIE Proceedings Vol. 5630:
Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II
Britton Chance; Mingzhe Chen; Arthur E. T. Chiou; Qingming Luo, Editor(s)

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