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Proceedings Paper

Cytological effect of laser-irradiated peanut seeds and induction of mutation
Author(s): Lin Ou; Rong Chen; Tong Lin; Buhong Li; Weijian Zhuang
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Paper Abstract

In this present paper, dry seeds of peanut of cytological effects and agro-character in F1 generation have been studied for YAG laser-irradiated at 1060 nm. With transmission electron microscope, the effects of ultrastructure of plumule cell were observed. There were difference in the cell on membrane system, fatbody and nucleus, especially in proteinbody, and there was chromosomal aberration of peanut root tip cell induced with laser 35.84w/cm2 power density. With irradiating time added from3s to 8s the rate of aberration increased from 0.16% up to 0.53%, and the types of chromosomal aberration also increased. Many aberration types, such as earlier separated mitosis metaphase, variability of number, sticky, non-equal division, polypolarity division, nucleus protrudes to cytoplasm and so on were found in 8s treatment but not in 3s. However, the dose of 8s treatment leads to lethal dose according to the seeds sprouting and growing. The seeds with 3s treatment, mine stem height, total pods per plant, mature pods per plant and weight per plant were more than the seeds unirradiated.

Paper Details

Date Published: 18 January 2005
PDF: 5 pages
Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); doi: 10.1117/12.572813
Show Author Affiliations
Lin Ou, Fujian Normal Univ. (China)
Rong Chen, Fujian Normal Univ. (China)
Tong Lin, Fujian Normal Univ. (China)
Buhong Li, Fujian Normal Univ. (China)
Weijian Zhuang, Fujian Agriculture and Forest Univ. (China)

Published in SPIE Proceedings Vol. 5630:
Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II
Britton Chance; Mingzhe Chen; Arthur E. T. Chiou; Qingming Luo, Editor(s)

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