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Proceedings Paper

Subwavelength-resolution fluorescent correlation spectroscopy using a solid immersion lens
Author(s): Alexandre Serov; Ramachandra G. Rao; Michael Gosch; Tiemo Anhut; Dietrich Martin; Robert Brunner; Rudolf Rigler; Theo Lasser
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Paper Abstract

In this paper we present recent spectroscopic studies using a Solid Immersion Lens for Fluorescent Correlation Spectroscopy measurements. We compare the performance of the Solid Immersion Lens confocal microscope built-up in our group to the performance of a conventional confocal microscope used for FCS. The novelty of the new SIL-FCS microscope is a system containing a conventional objective (NA = 0.6) combined with a Solid Immersion Lens used for single molecule experiment. Important parameters for single molecule experiments such as collection efficiency and excitation field confinement are investigated for different modes of the SIL objective system.

Paper Details

Date Published: 29 July 2004
PDF: 8 pages
Proc. SPIE 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, (29 July 2004); doi: 10.1117/12.572046
Show Author Affiliations
Alexandre Serov, Swiss Federal Institute of Technology Lasusanne (Switzerland)
Ramachandra G. Rao, Swiss Federal Institute of Technology Lausanne (Switzerland)
Michael Gosch, Karolinska Institute (Sweden)
Tiemo Anhut, Swiss Federal Institute of Technology Lausanne (Switzerland)
Dietrich Martin, Carl Zeiss GmbH (Germany)
Robert Brunner, Carl Zeiss GmbH (Germany)
Rudolf Rigler, Karolinska Institute (Sweden)
Theo Lasser, Swiss Federal Institute of Technology Lausanne (Switzerland)


Published in SPIE Proceedings Vol. 5486:
ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics
Ruikang K. Wang; Jeremy C. Hebden; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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