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Proceedings Paper

The nonlinear imaging theory of second harmonic and third harmonic generations in confocal microscopy
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Paper Abstract

The nonlinear imaging theory of second harmonic generation (SHG) and third harmonic generation (THG) in confocal microscopy is presented in this paper. The nonlinear effect of SHG and THG on the imaging properties of confocal microscopy has been analyzed in detail by these imaging theory. It is proved that the imaging process of SHG and THG in confocal microscopy, which is different from conventional coherent imaging or incoherent imaging, can be divided into two different processes of coherent imaging. The three-dimensional point spread functions (3D-PSF) of SHG and THG confocal microscopy are derived based on the nonlinear principles of SHG and THG. The imaging properties of SHG and THG confocal microscopy are discussed in detail according to its 3D-PSF. It is showed that the resolution of SHG and THG confocal microscopy are higher than that of single- and two-photon confocal microscopy.

Paper Details

Date Published: 29 July 2004
PDF: 8 pages
Proc. SPIE 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, (29 July 2004); doi: 10.1117/12.572025
Show Author Affiliations
Zhi-lie Tang, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Song-hao Liu, South China Normal Univ. (China)


Published in SPIE Proceedings Vol. 5486:
ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics
Ruikang K. Wang; Jeremy C. Hebden; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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