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Proceedings Paper

Determination of multiple components in urine using FT-MIR, NIR, and FT-Raman spectroscopic technique
Author(s): Weiling Liu; Kexin Xu; Qilian Yu; Sixiang Zhang; Duogang Ran
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Paper Abstract

This paper is centered on the development of reagent-free method based on Fourier transform mid infrared (FT-MIR), near infrared red (NIR) and Fourier transform (FT)-Raman spectroscopy. Suitable spectral wavenumber regions were selected for principal least square (PLS) regression and calibration models developed. In MIR, urine sample measured with Horizontal ATR, which is the accessory of Perkin-Elmer Spectrum GX Spectrometer, and the important range is 900-1800 cm-1. NIR spectra are obtained for each sample with 1mm pathlength. Absorption spectra of urine samples in 5000~4200cm-1 is used for PLS regression. The Raman spectra were recorded with a Bruker IFS 66 spectrometer with an FRA 106 FT-Raman accessory unit. The FT-Raman spectra of albumin power and albumin solution is recorded. The main noise is form water. The root mean square error of prediction (RMSEP) of glucose is 34.9 mg/dL(MIR), 31.56mg/dL(NIR) , and the RMSEP of albumin is 23.2mg/dL (MIR), 17.9mg/dL(NIR). Result indicated that FT-MIR and NIR spectroscopy could be used for rapid analysis of urine. FT-Raman spectroscopy could applied for compositional identification.

Paper Details

Date Published: 10 January 2005
PDF: 6 pages
Proc. SPIE 5640, Infrared Components and Their Applications, (10 January 2005); doi: 10.1117/12.571816
Show Author Affiliations
Weiling Liu, Tianjin Univ. (China)
Hebei Univ. of Technology (China)
Kexin Xu, Tianjin Univ. (China)
Qilian Yu, Tianjin Univ. (China)
Sixiang Zhang, Hebei Univ. of Technology (China)
Duogang Ran, Hebei Univ. of Technology (China)

Published in SPIE Proceedings Vol. 5640:
Infrared Components and Their Applications
Haimei Gong; Yi Cai; Jean-Pierre Chatard, Editor(s)

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