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Proceedings Paper

Raman molecular chemical imaging: 3D Raman using deconvolution
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Paper Abstract

Chemical imaging is a powerful technique combining molecular spectroscopy and digital imaging for rapid, non-invasive and reagentless analysis of materials, including biological cells and tissues. Raman chemical imaging is suited to the characterization of molecular composition and structure of biomateials at submicron spatial resolution (< 250 nm). As a result, Raman imaging has potential as a routine tool for the assessment of cells and subcellular components. In this presentation, we discuss Raman chemical imaging and spectroscopy of single human cells obtained from a culture line. Rapid three dimensional Raman imaging is shown using deconvolution to improve image quality.

Paper Details

Date Published: 7 December 2004
PDF: 8 pages
Proc. SPIE 5588, Smart Medical and Biomedical Sensor Technology II, (7 December 2004); doi: 10.1117/12.571512
Show Author Affiliations
John S. Maier, Chemimage Corp. (United States)
Patrick J. Treado, Chemimage Corp. (United States)

Published in SPIE Proceedings Vol. 5588:
Smart Medical and Biomedical Sensor Technology II
Brian M. Cullum, Editor(s)

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