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Proceedings Paper

Development of a MEMS 2D separations device
Author(s): Kristen Perlot Bloschock; Jonathan N. Flyer; Thomas W. Schneider; Abul Hussam; Edward R. Van Keuren
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Paper Abstract

A polymer based biochip for rapid 2D separations of peptides, proteins, and other biomedically relevant molecules was designed and fabricated. Like traditional 2D polyacrylamide gel electrophoresis (2D-PAGE) methods, the device will allow molecules to separate based on isoelectric point (pI) and molecular weight (MW). Our design, however, integrates both an initial capillary isoelectric focusing (cIEF) step followed by capillary electrophoresis (CE) in multiple parallel channels, all on a single microfluidic chip. Not only is the "lab-on-a-chip" design easier to use and less expensive, but the miniaturization of the device produces very rapid separations. Compared to traditional 2D-PAGE, which can take hours to complete, we estimate separation times on the order of seconds. Fluorescence detection will be used in the preliminary stages of testing, but the device also is equipped with integrated electrodes in the electrophoresis channels to perform multiplexed electrochemical detection for quantitative analysis. We will present preliminary results of the chip development and testing.

Paper Details

Date Published: 8 December 2004
PDF: 8 pages
Proc. SPIE 5591, Lab-on-a-Chip: Platforms, Devices, and Applications, (8 December 2004); doi: 10.1117/12.571466
Show Author Affiliations
Kristen Perlot Bloschock, Georgetown Univ. (United States)
Jonathan N. Flyer, Georgetown Univ. (United States)
Thomas W. Schneider, Science Applications International Corp. (United States)
Abul Hussam, George Mason Univ. (United States)
Edward R. Van Keuren, Georgetown Univ. (United States)

Published in SPIE Proceedings Vol. 5591:
Lab-on-a-Chip: Platforms, Devices, and Applications
Linda A. Smith; Daniel Sobek, Editor(s)

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