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Proceedings Paper

Automatic enhancement of skin fluorescence localization due to refractive index matching
Author(s): Dmitry Y. Churmakov; Igor Vladislavovich Meglinski; Sergey A. Piletsky; Douglas A. Greenhalgh
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Paper Abstract

Fluorescence diagnostic techniques are notable amongst many other optical methods, as they offer high sensitivity and non-invasive measurements of tissue properties. However, a combination of multiple scattering and physical heterogeneity of biological tissues hampers the interpretation of the fluorescence measurements. The analyses of the spatial distribution of endogenous and exogenous fluorophores excitations within tissues and their contribution to the detected signal localization are essential for many applications. We have developed a novel Monte Carlo technique that gives a graphical perception of how the excitation and fluorescence detected signal are localized in tissues. Our model takes into account spatial distribution of fluorophores and their quantum yields. We demonstrate that matching of the refractive indices of ambient medium and topical skin layer improves spatial localization of the detected fluorescence signal within the tissue. This result is consistent with the recent conclusion that administering biocompatible agents results in higher image contrast.

Paper Details

Date Published: 29 July 2004
PDF: 12 pages
Proc. SPIE 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, (29 July 2004); doi: 10.1117/12.571358
Show Author Affiliations
Dmitry Y. Churmakov, Cranfield Univ. (United Kingdom)
Igor Vladislavovich Meglinski, Cranfield Univ. (United Kingdom)
Sergey A. Piletsky, Cranfield Univ. (United Kingdom)
Douglas A. Greenhalgh, Cranfield Univ. (United Kingdom)


Published in SPIE Proceedings Vol. 5486:
ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics
Ruikang K. Wang; Jeremy C. Hebden; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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