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Multiple UV wavelength excitation and fluorescence of bioaerosols
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Paper Abstract

A two-wavelength excitation bioaerosol sensor has been developed and characterized for classifying various types of aerosols, including biological organisms and non-biological interferents. Single aerosols, smaller than 10 μm, are interrogated with 266 nm and 355 nm laser pulses separated in time by 400 ns. Fluorescence signals excited by these pulses are detected in three broad spectral bands centered at 350 nm, 450 nm and 550 nm. The results indicate that bacterial spores, vegetative bacterial cells and proteins can be differentiated based on the two wavelength excitation approach.

Paper Details

Date Published: 16 December 2004
PDF: 8 pages
Proc. SPIE 5585, Chemical and Biological Point Sensors for Homeland Defense II, (16 December 2004); doi: 10.1117/12.571296
Show Author Affiliations
Vasanthi Sivaprakasam, Naval Research Lab. (United States)
Alan L. Huston, Naval Research Lab. (United States)
Cathy S. Scotto, Naval Research Lab. (United States)
Jay D. Eversole, Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 5585:
Chemical and Biological Point Sensors for Homeland Defense II
Arthur J. Sedlacek III; Steven D. Christesen; Tuan Vo-Dinh; Roger J. Combs, Editor(s)

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