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Proceedings Paper

Design of a portable fluorometer capable of time-resolved luminescence measurements
Author(s): Guoying Chen
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Paper Abstract

A rugged, filter-based fluorometer capable of time-resolved luminescence (TRL) measurements was designed, prototyped and tested for field applications. The instrument operation and data processing were controlled by a laptop computer running a custom LabVIEW program. A xenon flashlamp was used as the light source and a photomultiplier tube (PMT) as the photodetector. A gating technique was implemented to effectively overcome PMT saturation by intense xenon lamp flash so signal integrity was maintained even at very high gains, leading to improved sensitivity and reproducibility. The instrument was tested by TRL using tetracycline as a model analyte; and the signal was digitized at a 2-μs time resolution and a 12-bit amplitude resolution. Its performance was similar to or slightly better than that of a commercial fluorescence spectrophotometer. A 0-300 ppb linear dynamic range (r2 = 0.9996) and a 0.025-ppb limit of detection (LOD) were achieved with a ≤5% relative standard deviation.

Paper Details

Date Published: 19 November 2004
PDF: 7 pages
Proc. SPIE 5587, Nondestructive Sensing for Food Safety, Quality, and Natural Resources, (19 November 2004); doi: 10.1117/12.570527
Show Author Affiliations
Guoying Chen, U.S. Dept. of Agriculture (United States)


Published in SPIE Proceedings Vol. 5587:
Nondestructive Sensing for Food Safety, Quality, and Natural Resources
Yud-Ren Chen; Shu-I Tu, Editor(s)

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