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Proceedings Paper

H-Bond network in biological systems: an infrared study
Author(s): Joze Grdadolnik
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Paper Abstract

A stepwise hydration of bovine serum albumin is studied using infrared spectroscopy. For determination of the configuration interactions of hydrating H20 molecules to specific protein parts evaporation spectra were used. Evaporation spectra were calculated by subtraction of the spectra of hydrated protein from the spectrum of the dry protein. Evaporation spectra are further processed with a novel method, which is based on the decomposition of the original spectra into basic vectors (spectra). For detailed assignment of the basic spectra the temperature measurements and H/D exchanged were used. Upon the assignment of the basic vectors several interaction configurations of H20 molecules attached to specific protein sites as a function of the hydration level were proposed.

Paper Details

Date Published: 20 July 2004
PDF: 10 pages
Proc. SPIE 5507, XVI International Conference on Spectroscopy of Molecules and Crystals, (20 July 2004); doi: 10.1117/12.570003
Show Author Affiliations
Joze Grdadolnik, National Institute of Chemistry (Slovenia)

Published in SPIE Proceedings Vol. 5507:
XVI International Conference on Spectroscopy of Molecules and Crystals
Galyna O. Puchkovska; Tatiana A. Gavrilko; Olexandr I. Lizengevich, Editor(s)

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