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Proceedings Paper

Vibrational spectroscopy and principal component analysis for conformational study of virus nucleic acids
Author(s): G. I. Dovbeshko; O. P. Repnytska; T. Pererva; A. Miruta; D. Kosenkov
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Paper Abstract

Conformation analysis of mutated DNA-bacteriophages (PLys-23, P23-2, P47- the numbers have been assigned by T. Pererva) induced by MS2 virus incorporated in Ecoli AB 259 Hfr 3000 has been done. Surface enhanced infrared absorption (SEIRA) spectroscopy and principal component analysis has been applied for solving this problem. The nucleic acids isolated from the mutated phages had a form of double stranded DNA with different modifications. The nucleic acid from phage P47 was undergone the structural rearrangement in the most degree. The shape and position ofthe fine structure of the Phosphate asymmetrical band at 1071cm-1 as well as the stretching OH vibration at 3370-3390 cm-1 has indicated to the appearance ofadditional OH-groups. The Z-form feature has been found in the base vibration region (1694 cm-1) and the sugar region (932 cm-1). A supposition about modification of structure of DNA by Z-fragments for P47 phage has been proposed. The P23-2 and PLys-23 phages have showed the numerous minor structural changes also. On the basis of SEIRA spectra we have determined the characteristic parameters of the marker bands of nucleic acid used for construction of principal components. Contribution of different spectral parameters of nucleic acids to principal components has been estimated.

Paper Details

Date Published: 20 July 2004
PDF: 8 pages
Proc. SPIE 5507, XVI International Conference on Spectroscopy of Molecules and Crystals, (20 July 2004); doi: 10.1117/12.570000
Show Author Affiliations
G. I. Dovbeshko, Institute of Physics (Ukraine)
O. P. Repnytska, Institute of Physics (Ukraine)
T. Pererva, Institute of Molecular Biology and Genetics (Ukraine)
A. Miruta, Institute of Molecular Biology and Genetics (Ukraine)
D. Kosenkov, Taras Shevchenko National Univ. of Kyiv (Ukraine)

Published in SPIE Proceedings Vol. 5507:
XVI International Conference on Spectroscopy of Molecules and Crystals
Galyna O. Puchkovska; Tatiana A. Gavrilko; Olexandr I. Lizengevich, Editor(s)

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